IgG1 IgG2a IgG2b IgG3 (Fc specific) polyclonal, anti-mouse

Quantity: 200 µl

Background:
The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. The antiserum contains antibodies to subclass-specific determinants as well as to determinants shared by two or more subclasses of IgG. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal mouse plasma, serum, and immunoglobulin fractions, no reaction is obtained with IgA, IgM and IgG/Fab fragments or any other serum protein. In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal mouse serum; to prepare an immunoadsorbent for the production of Ig-free mouse serum, plasma or non-Ig preparations.

Isotype: Goat Polyclonal

Source: Purified normal IgG, including all known subclasses, isolated from pooled mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Delipidated, heat inactivated, in PBS and 0.1% sodium azide.

Specificity:
Precipitating polyclonal Goat anti-Serum to Mouse IgG, Fc specific

Reactivity: mouse

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has been tested in double radial immunodiffusion anti several sera with the following results: cow - Duck - Horse - Rat + Cat - Guinea Pig+ Monkey - Sheep - Chicken - Human - pigeon - Swine - Dog - Hamster ﾱ Rabbit - Turkey -

Applications: Immunoprecipitation

Storage: Store at 4C, or in small aliquots at -20C.