IgG1 IgG2a IgG2b IgG3 (Fc specific) polyclonal, anti-mouse

Quantity: 1 ml

Background:
The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. The antiserum contains antibodies to subclass-specific determinants as well as to determinants shared by two or more subclasses of IgG. In immunoelectrophoresis and double radial immunodiffusion, using various anti- serum concentrations against normal mouse plasma, serum, and immunoglobulin fractions, no reaction is obtained with IgA, IgM and IgG/Fab fragments or any other serum protein. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non- isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:100 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5,000.

Isotype: Rabbit Polyclonal

Source: Purified normal IgG, including all known subclasses, isolated from pooled mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Purified hyperimmune rabbit IgG, in phosphate buffered saline (PBS, pH 7.2).

Reactivity: mouse

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has been tested in double radial immunodiffusion anti several sera with the following results: Bovine - Cat - Chicken - Dog - Duck - Goat - Guinea Pig+ Human - Hamster + Horse - Monkey - pigeon - Rat + Sheep - Swine - Turkey - The absence of a reaction in double radial immunodiffusion does not exclude some reaction in more sensitive techniques.

Applications: Indirect immunofluorescence, ELISA, Dot blot, Immunoblotting

Storage: Store at 4C, or in small aliquots at -20C.