IgG1 IgG2a IgG2b IgG3 (Fc specific) polyclonal HRP, anti-mouse

Quantity: 200 µl

Background:
The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule which expresses strict isotypic (class) specificity as tested against mouse serum and a panel of purified homogenous immunoglobulins In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in mouse serum or other body fluids; in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgG in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:10,000 depending on the method used.

Isotype: Rabbit Polyclonal

Source: Purified homogenous IgG isolated from pooled mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Peroxidase-coupled purified hyperimmune rabbit IgG, in phosphate buffered saline (PBS, pH 7.2).

Reactivity: mouse

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has been tested in double radial immunodiffusion anti several species sera with the following results: Bovine - Cat - Chicken - Dog - Duck - Goat - Guinea Pig + Hamster + Horse - Human - Monkey - pigeon - Rat + Sheep - Swine - Turkey - The absence of a reaction in double radial immunodiffusion does not exclude some reaction in more sensitive techniques.

Applications: ELISA, Immunocytochemistry, Immunohistochemistry (paraffin), Dot blot, Immunoblotting

Storage: Store at 4C, or in small aliquots at -20C.