IgG1 IgG2a IgG2b IgG3 IgA IgM (heavy and light chains) polyclonal, anti-mouse

Quantity: 1 ml

Background:
The defined antibody specificity is directed to the major isotypes of the mouse immunoglobulin system (classes, subclasses and L chain types) including antibody to common determinants, to class and subclassspecific determinants, and to the surface determinants of the common Fab portion as tested against as tested in immunoelectrophoresis and double radial immunodiffusion (Ouchterlony). The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays are between 1:500 and 1:5,000.

Isotype: Rabbit Polyclonal

Source: Highly purified IgG, including all subclasses, and pools of homogenous IgA and IgM isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure

Form: Purified hyperimmune rabbit IgG, in phosphate buffered saline (PBS, pH 7.2).

Reactivity: mouse

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to mammalian immunoglobulins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. If the product is to be used in the presence of material originating from an other species than Human, prior screening for cross-reactivity with that material is essential and additional adsorption of the reagent may be required to ensure intra-assay specificity.

Applications: Indirect immunofluorescence, ELISA, Dot blot, Immunoblotting

Storage: Store at 4C, or in small aliquots at -20C.