IgG1 IgG2a IgG2b IgG3 IgA IgM (heavy and light chains) polyclonal HRP, anti-mouse

Quantity: 200 µl

Background:
The reactivity of the antiserum is directed to the major isotypes of the mouse immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal mouse plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. In enzyme-immunocytochemical and immunohistochemical staining for the detection of cytoplasmic Ig at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases.This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:2,000 and 1:10,000.

Isotype: Goat Polyclonal

Source: Purified polyclonal mouse IgG, and homogenous IgA and IgM isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Peroxidase-coupled purified hyperimmune goat IgG, in phosphate buffered saline (PBS, pH 7.2).

Specificity:
Horseradish peroxidase–conjugated IgG fraction of polyclonal Goat anti-Serum to Mouse IgG, IgA and IgM, heavy and light chains

Reactivity: mouse

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to mammalian immunoglobulins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiserum fraction has been tested for cross-reactivity by double radial immunodiffusion anti several species sera with the following results:Bovine - Duck - Human - Rat + Cat - Guinea Pig + Monkey - Sheep - Chicken - Hamster � pigeon - Swine - Dog - Horse - Rabbit - Turkey -

Applications: ELISA, Immunocytochemistry, Immunohistochemistry (paraffin), Dot blot, Immunoblotting

Storage: Store at 4C, or in small aliquots at -20C.