IgG1 IgG2a IgG2b IgG3 IgA IgM IgD IgE (heavy and light chains) polyclonal, anti-mouse

Quantity: 200 µl

Background:
The reactivity of the antiserum is directed to the heavy chains of immunoglobulin isotypes IgG, IgA, IgM, IgD and IgE. The antiserum contains antibodies to common determinants and to the surface determinants of the common Fab as tested by immunoelectrophoresis and double radial immunodiffusion. It does not react with any non-Ig proteins in mouse serum. In immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of immunoglobulins in mouse serum or other body fluids; to prepare an immunoadsorbent for the purification of mouse immunoglobulins from serum or plasma; as secondary antibody to precipitate the immunoglobulin in normal mouse serum. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.

Isotype: Rabbit Polyclonal

Source: Purified polyclonal mouse IgG and pools of homogenous IgG, IgA, IgM, IgD and IgE. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Delipidated, heat inactivated, in PBS and 0.1% sodium azide.

Reactivity: mouse

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

Applications: Immunoprecipitation

Storage: Store at 4C, or in small aliquots at -20C.