IgG1 IgG2a IgG2b IgG3 IgA IgM polyclonal, anti-mouse

Quantity: 200 µl

Background:
The reactivity of the antiserum is directed the major immunoglobulin isotypes of the mouse Ig system (classes, subclasses and L-chain types), including antibodies to common determinants, to class and subclass-specific determinants, and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal mouse plasma and serum. In immunocytochemical and immunohistochemical staining of immunoglobulins at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure immunoglobulins in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:2,000 and 1:20,000.

Isotype: Rabbit Polyclonal

Source: Purified polyclonal IgG and pools of homogenous IgA and IgM isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Biotin-coupled hyperimmune goat IgG, in phosphate buffered saline (PBS, pH 7.2).

Reactivity: mouse

Cross Reactivity:
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. The antiserum has been tested for cross-reactivity in double radial immunodiffusion with the following results: Bovine + Duck - Horse + Rat ++ Cat + Goat + Human + Sheep + Chicken - Guinea Pig ++ Monkey + Swine + Dog + Hamster ++ pigeon - Turkey ﾱ A negative cross-reaction in double radial immunodiffusion does not exclude some reaction in more sensitive techniques.

Applications: ELISA, Immunocytochemistry, Immunohistochemistry (paraffin), Dot blot, Immunoblotting

Storage: Store at 4C, or in small aliquots at -20C.