IgG2a (subclass specific) polyclonal, anti-rat

Quantity: 200 µl

Background:
The reactivity of the antiserum is directed to the Fc portion of the subclass IgG2a. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG2a in rat serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of rat IgG2a from serum or plasma.

Isotype: Rabbit Polyclonal

Source: Pools of purified homogenous IgG2a isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Delipidated, heat inactivated, in PBS and 0.1% sodium azide.

Reactivity: rat

Cross Reactivity:
This IgG fraction is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this product has not been tested in detail, however in double radial immunodiffusion a weak reaction with Mouse has been observed.

Applications: Immunoprecipitation

Storage: Store at 4C, or in small aliquots at -20C.