IgG2ab (subclass specific) polyclonal, anti-mouse

Quantity: 200 µl

Background:
The reactivity of the antiserum is directed to the subclasses IgG2a and IgG2b.The antiserum contains antibodies to iso and allospecific determinants. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG2 in mouse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of mouse IgG2 from serum or plasma.

Isotype: Goat Polyclonal

Source: Pools of purified homogenous IgG2a and IgG2b isolated from mouse serum of strains belonging to the allelic types Igh-1a and Igh-1b. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Delipidated, heat inactivated, in PBS and 0.1% sodium azide.

Specificity:
Precipitating polyclonal Goat anti-Serum to Mouse IgG2a, subclass specific

Reactivity: mouse

Cross Reactivity:
This IgG fraction is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this product has not been tested in detail.

Applications: Immunoprecipitation

Storage: Store at 4C, or in small aliquots at -20C.