IgG2c (subclass specific) polyclonal TRITC, anti-rat

Quantity: 200 µl

Background:
The reactivity of the antiserum is directed to the subclass IgG2c. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining for the detection of IgG2c at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgG2c in rat serum or other body fluids.This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.Working dilutions are usually between 1:20 and 1:80, depending on the method used.

Isotype: Goat Polyclonal

Source: Pools of purified homogenous IgG2c isolated from rat sera. Freund's complete adjuvant is used in he first step of the immunization procedure.

Form: TRITC-coupled purified hyperimmune goat IgG, in phosphate buffered saline (PBS, pH 7.2)

Specificity:
Tetramethylrhodamine isothiocyanate-conjugated IgG fraction of polyclonal Goat anti-Serum to Rat IgG2c, subclass specific.

Reactivity: rat

Cross Reactivity:
This immunoconjugate is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this immunoconjugate has not been tested in detail.

Applications: ELISA, Immunocytochemistry, Immunohistochemistry (frozen), (In)direct immunofluorescence

Storage: Store at 4C, or in small aliquots at -20C.