IgM (Fc specific) polyclonal, anti-sheep

Quantity: 200 µl

Background:
The reactivity of the antiserum is restricted to the Fc part of the IgM molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal sheep serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgM. No precipitation reaction is obtained with purified IgG, IgA, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in sheep serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of sheep IgM from serum or plasma.

Isotype: Rabbit Polyclonal

Source: Highly purified normal IgM isolated from pooled sheep serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Form: Delipidated, heat inactivated, in PBS and 0.1% sodium azide.

Reactivity: Sheep

Cross Reactivity:
The antiserum does not cross-react with any other component of the Sheep Ig system. Interspecies cross reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail, however in double radial immunodiffusion a strong reaction with Goat has been observed.

Applications: Immunoprecipitation

Storage: Store at 4C, or in small aliquots at -20C.