IKBKE polyclonal, anti-human

Background:
The transcription factor NFκB is retained in the cytoplasm in an inactive form by the inhibitory protein IκB. Activation of NFκB requires that IκB be phosphorylated on specific serine residues, which results in targeted degradation of IκB. IκB kinase α (IKKα), previously designated CHUK, interacts with IκB-α and specifically phosphorylates IκB-α on the sites that trigger its degradation, Serines 32 and 36. The functional IKK complex contains three subunits, IKKα, IKKβ and IKKγ (also designated NEMO), and each appear to make essential contributions to IκB phosphorylation. IKK-i is a serine/threonine kinase that shares homology with IKKα and IKKβ. IKK-i is primarily expressed in immune cells and is induced by lipopolysaccharide and by proinflammatory cytokines including TNFα, IL-1 and IL-6. Overexpression of IKK-i has been shown to result in phosphorylation of IκBα on Ser 32 and Ser 36, and in NFκB activation, suggesting that IKK-i may act as an IκB kinase in the immune system.

Alternative Name:
I-kappa-B kinase epsilon, IKK-E, IKK-epsilon, IkBKE, IKK-i, IKBKE, IKKE, IKKI, KIAA0151, I kappa B kinase epsilon, I-kappa-B kinase epsilon, IkBKE, IKK related kinase epsilon, IKK-E, IKK-epsilon, IKK-i, IKKE, IKKE, IKKepsilon, IKKI, Inducible I kappa B kinase, Inducible I kappa-B kinase, Inducible IkappaB kinase, Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon, Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, epsilon, Inhibitor of nuclear factor kappa-B kinase subunit epsilon, KIAA0151, MGC125294, MGC125295, MGC125297,

Application Dilution:
WB:1:500-1:2,000
IHC/ICC:1:50-1:200
FC:1:50-1:100

Specificity: IKBKE polyclonal antibody detects endogenous levels of IKBKE protein.

Immunogen:
Recombinant protein

MW: 80 kDa

Swiss-Prot: Q14164(Human)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.