IKK-alpha (Phospho-Ser176) /IKK-beta (Phospho-Ser177) polyclonal, anti-human, mouse, rat

Specificity:
IKK-alpha (Phospho-Ser176)/IKK-beta (Phospho-Ser177) Antibody detects endogenous levels of CHUK/IKBKB only when IKK-alpha phosphorylated at Ser176 and IKK-beta phosphorylated at Ser177.

Synonyms: CHUK, Conserved helix-loop-helix ubiquitous kinase, I kappa-B kinase alpha, I-kappa-B kinase 1, IkappaB kinase, IkBKA, IKK-A, IKK-alpha, IKK1, IKKA, Inhibitor of nuclear factor kappa-B kinase alpha subunit, kinase IKK-alpha, NFKBIKA, Nuclear factor NFkapp

Immunogen:
The antiserum was produced against synthesized phospho-peptide derived from human IKK-alpha around the phosphorylation site of Ser176 and IKK-beta around the phosphorylation site of Ser177.

Immunogen Sequence:
Phospho

Modification Sites: H:S176/S177, M:S176/S177, R:S176/S177

MW (kDa): 84 kDa

NCBI Gene Symbol: CHUK/IKBKB

Database Links:
Gene ID: 1147/3551
Swiss Prot No.: O15111/O14920
OMIM No.: 600664/603258
Unigene No.: Hs.198998/Hs.656458

Application Dilutions: WB: 1:500~1:1000, IHC: 1:50~1:100, ELISA: 1:10000

Purification:
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Physical Format:
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Concentration: 1 mg/ml

Storage: Stable at -20°C for at least 1 year.