IKKα/β polyclonal, anti-human, mouse, rat

Background:
The transcription factor NFkB is retained in the cytoplasm in an inactive form by the inhibitory protein IkB. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase a (IKKa), previously designated CHUK, interacts with IkB-a and specifically phosphorylates IkB-a on Ser 32 and 36, the sites that trigger its degradation. IKKa appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKKa is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKa, IKKb and IKKg (also designated NEMO), and each appear to make essential contributions to IkB phosphorylation.

Alternative Name:
I-kappa-B kinase alpha, IKK-A, IKK-alpha, IkBKA, IkappaB kinase, IKK1, NFKBIKA, TCF-16, CHUK, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, IKK2, NFKBIKB, IKBKB, CHUK, Conserved helix loop helix ubiquitous kinase, I kappa B kinase 1, I kappa B kinase 2, I Kappa B kinase alpha, I Kappa B kinase beta, IkB kinase alpha subunit, IkBKA, IkBKB, IKK a kinase, IKK alpha, IKK beta, IKK1, IKK2, IKKA, IKKB, IMD15, Inhibitor of kappa light polypeptide gene enhancer in B cells kinase beta, Inhibitor of nuclear factor kappa-B kinase subunit alpha, Inhibitor of nuclear factor kappa-B kinase subunit beta, NFKBIKA, NFKBIKB, Nuclear factor NF kappa B inhibitor kinase beta, Nuclear factor NFkappaB inhibitor kinase alpha, TCF 16, TCF16, Transcription factor 16,

Application Dilution:
WB:1:1,000
ICC:1:100-1:500

Specificity: IKKα/β polyclonal antibody detects endogenous levels of IKKα/β protein.

Immunogen:
recombinant protein

MW: 85/87 kDa

Swiss-Prot: O14920(Human) O15111(Human) O88351(Mouse) Q60680(Mouse) Q9QY78(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.