IL-10 monoclonal, anti-rat
€0.00
In stock
SKU
AC-ABG10196
Catalog Number: AC-ABG10196
Size(s): 500 µg
Host: Mouse
Applications: WB, E
Datasheet
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Size(s): 500 µg
Host: Mouse
Applications: WB, E
Datasheet
Request Information
Source:
Produced in BALB/c mice using highly pure recombinant Rat IL-10 as the immunizing antigen. This antibody was purified from tissue culture by Protein A affinity chromatography.
Western Blot:
To detect Rat IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-10 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.
Sandwich ELISA:
In a sandwich ELISA (assuming 100μl/well), a concentration of 8.0-16.0 μg/ml of this antibody will detect at least 500 pg/ml of recombinant Rat IL-10 when used with BioGems Biotinylated Antigen Affinity Purified Anti-Rat IL-10 (62-010BT) as the detection antibody at a concentration of approximately 0.5-1.0 μg/ml.
Formulation: A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Produced in BALB/c mice using highly pure recombinant Rat IL-10 as the immunizing antigen. This antibody was purified from tissue culture by Protein A affinity chromatography.
Western Blot:
To detect Rat IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-10 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.
Sandwich ELISA:
In a sandwich ELISA (assuming 100μl/well), a concentration of 8.0-16.0 μg/ml of this antibody will detect at least 500 pg/ml of recombinant Rat IL-10 when used with BioGems Biotinylated Antigen Affinity Purified Anti-Rat IL-10 (62-010BT) as the detection antibody at a concentration of approximately 0.5-1.0 μg/ml.
Formulation: A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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