IL-11 monoclonal, anti-human
€0.00
In stock
SKU
AC-ABG10199
Catalog Number: AC-ABG10199
Size(s): 500 µg
Host: Mouse
Applications: WB, IHC, E
Datasheet
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Size(s): 500 µg
Host: Mouse
Applications: WB, IHC, E
Datasheet
Request Information
Source:
Produced in BALB/c x ICR F1 mice using highly pure (>98%) recombinant human IL-11 as the immunizing antigen. This IgG1k antibody was purified from cell culture by Protein G affinity chromatography.
Western Blot:
To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 0.25-0.50 ng/lane, under non-reducing conditions.
Sandwich ELISA:
In a sandwich ELISA (assuming 100 μl/well), a concentration of 8.0-9.0 μg/ml of this antibody will detect recombinant human IL-11 when used with BioGems biotinylated antigen affinity purified anti-human IL-11 (60-011BT) as the detection antibody at a concentration of approximately 2.0-4.0 μg/ml.
Immunohistochemistry:
This antibody stained human brain stroke (including control cortex and stroke core areas) tissue. The primary antibody was incubated at 2.5 μg/ml overnight at 4˚C. This was followed by a fluorophore conjugated secondary antibody. Optimal concentrations and conditions may vary.
Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm.
Formulation: A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Produced in BALB/c x ICR F1 mice using highly pure (>98%) recombinant human IL-11 as the immunizing antigen. This IgG1k antibody was purified from cell culture by Protein G affinity chromatography.
Western Blot:
To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 0.25-0.50 ng/lane, under non-reducing conditions.
Sandwich ELISA:
In a sandwich ELISA (assuming 100 μl/well), a concentration of 8.0-9.0 μg/ml of this antibody will detect recombinant human IL-11 when used with BioGems biotinylated antigen affinity purified anti-human IL-11 (60-011BT) as the detection antibody at a concentration of approximately 2.0-4.0 μg/ml.
Immunohistochemistry:
This antibody stained human brain stroke (including control cortex and stroke core areas) tissue. The primary antibody was incubated at 2.5 μg/ml overnight at 4˚C. This was followed by a fluorophore conjugated secondary antibody. Optimal concentrations and conditions may vary.
Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm.
Formulation: A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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