IL-12 monoclonal, anti-human
€0.00
In stock
SKU
AC-ABG10204
Catalog Number: AC-ABG10204
Size(s): 500 µg
Host: Mouse
Applications: WB, IHC, E
Datasheet
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Size(s): 500 µg
Host: Mouse
Applications: WB, IHC, E
Datasheet
Request Information
Source:
Produced in BALB/c x ICR F1 mice using highly pure (>98%) recombinant human IL-12 as the immunizing antigen. This IgG1k antibody was purified from ascites fluid by Protein A affinity chromatography.
Western Blot:
To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.50-1.0 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 0.5-1.0 ng/lane, under non-reducing conditions.
Sandwich ELISA:
In a sandwich ELISA (assuming 100 μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 1000 pg/ml of recombinant human IL-12 when used with BioGems biotinylated antigen affinity purified anti-human IL-12 (60-012BT) as the detection antibody at a concentration of approximately 4.0-8.0 μg/ml.
Immunohistochemistry:
This antibody stained PBMC. The primary antibody was incubated at 10.0 μg/ml for one hour at room temperature followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary.
Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia.
Formulation: A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Produced in BALB/c x ICR F1 mice using highly pure (>98%) recombinant human IL-12 as the immunizing antigen. This IgG1k antibody was purified from ascites fluid by Protein A affinity chromatography.
Western Blot:
To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.50-1.0 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 0.5-1.0 ng/lane, under non-reducing conditions.
Sandwich ELISA:
In a sandwich ELISA (assuming 100 μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 1000 pg/ml of recombinant human IL-12 when used with BioGems biotinylated antigen affinity purified anti-human IL-12 (60-012BT) as the detection antibody at a concentration of approximately 4.0-8.0 μg/ml.
Immunohistochemistry:
This antibody stained PBMC. The primary antibody was incubated at 10.0 μg/ml for one hour at room temperature followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary.
Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia.
Formulation: A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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