IL-12 polyclonal, anti-human
€0.00
In stock
SKU
AC-ABG10200
Catalog Number: AC-ABG10200
Size(s): 50 µg, 100 µg
Host: Goat
Applications: WB, E
Datasheet
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Size(s): 50 µg, 100 µg
Host: Goat
Applications: WB, E
Datasheet
Request Information
Source:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hIL12. Anti-Human IL-12 specific antibody was purified by affinity chromatography employing immobilized hIL12 matrix.
Western Blot:
To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich ELISA:
To detect hIL-12 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Human IL-12 (60-012BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-12.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-12 (0.3 ng/ml), a concentration of 0.012-0.02 μg/ml of this antibody is required.
Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hIL12. Anti-Human IL-12 specific antibody was purified by affinity chromatography employing immobilized hIL12 matrix.
Western Blot:
To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich ELISA:
To detect hIL-12 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Human IL-12 (60-012BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-12.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-12 (0.3 ng/ml), a concentration of 0.012-0.02 μg/ml of this antibody is required.
Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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