IL-15 monoclonal, anti-human
€0.00
In stock
SKU
AC-ABG10214
Catalog Number: AC-ABG10214
Size(s): 500 µg
Host: Mouse
Applications: WB, E
Datasheet
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Size(s): 500 µg
Host: Mouse
Applications: WB, E
Datasheet
Request Information
Source:
Produced in BALB/c x C57BL/6 F1 mice using highly pure (>98%) recombinant human IL-15 as the immunizing antigen. This IgG1k antibody was purified from ammonium sulfate precipitation followed by ion exchange chromatography.
Western Blot:
To detect hIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.50-1.0 μg/ml (non-reducing) or at a concentration of 2.0-4.0 μg/ml (reducing). Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-15 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
Sandwich ELISA:
In a sandwich ELISA (assuming 100 μl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 250 pg/ml of recombinant human IL-15 when used with BioGems biotinylated antigen affinity purified anti-human IL-15 (60-015BT) as the detection antibody at a concentration of approximately 2.0-4.0 μg/ml.
Formulation: A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Produced in BALB/c x C57BL/6 F1 mice using highly pure (>98%) recombinant human IL-15 as the immunizing antigen. This IgG1k antibody was purified from ammonium sulfate precipitation followed by ion exchange chromatography.
Western Blot:
To detect hIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.50-1.0 μg/ml (non-reducing) or at a concentration of 2.0-4.0 μg/ml (reducing). Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-15 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
Sandwich ELISA:
In a sandwich ELISA (assuming 100 μl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 250 pg/ml of recombinant human IL-15 when used with BioGems biotinylated antigen affinity purified anti-human IL-15 (60-015BT) as the detection antibody at a concentration of approximately 2.0-4.0 μg/ml.
Formulation: A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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