IL-1β polyclonal, anti-mouse
€0.00
In stock
SKU
AC-ABG10243
Catalog Number: AC-ABG10243
Size(s): 50 µg, 100 µg
Host: Rabbit
Applications: WB, IHC, E
Datasheet
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Size(s): 50 µg, 100 µg
Host: Rabbit
Applications: WB, IHC, E
Datasheet
Request Information
Source:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-1beta. Anti-Murine IL-1beta specific antibody was purified by affinity chromatography employing immobilized mIL-1beta matrix.
Western Blot:
To detect mIL-1beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich ELISA:
To detect mIL-1beta by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Murine IL-1beta (61-001BBT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-1beta.
Immunohistochemistry:
This antibody stained colchicine injected mouse brain (polymorph layer of the dentate gyrus) tissue. The primary antibody was incubated at 0.5 μg/ml overnight at 4˚C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA™) reagent. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-1beta (10 pg/ml), a concentration of 0.57-0.86 μg/ml of this antibody is required.
Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-1beta. Anti-Murine IL-1beta specific antibody was purified by affinity chromatography employing immobilized mIL-1beta matrix.
Western Blot:
To detect mIL-1beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich ELISA:
To detect mIL-1beta by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Murine IL-1beta (61-001BBT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-1beta.
Immunohistochemistry:
This antibody stained colchicine injected mouse brain (polymorph layer of the dentate gyrus) tissue. The primary antibody was incubated at 0.5 μg/ml overnight at 4˚C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA™) reagent. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-1beta (10 pg/ml), a concentration of 0.57-0.86 μg/ml of this antibody is required.
Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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