IL-21 human active recombinant protein
€295.00
In stock
SKU
600142
Protein Family: Cytokines
Pathway and Disease: Immune System, Signal Transduction, Signaling Molecules and Interaction
Alternate Names: interleukin-21, IL-21, Za11, IL21Description:
Interleukin-21 (IL-21), which is expressed by activated CD4(+) T cells, is the newest member of the common gamma-chain family of cytokines (IL-2, IL-4, IL-7, IL-9, IL-13, and IL-15). IL-21 receptor (IL-21R) is expressed on T, B, and NK cells. Within the B cell lineage, IL-21 regulates IgG1 production and cooperates with IL-4 for the production of multiple antibody classes in vivo. IL-21R has been shown to activate the Janus kinase/signal transducers and activators of transcription signaling pathway upon ligand binding. Initial studies have demonstrated that IL-21 has pleiotropic effects on the proliferation, differentiation, and effector functions of B, T, natural killer, and dendritic cells. Recombinant Human IL-21 produced in E. coli is a single non-glycosylated polypeptide chain containing 133 amino acids with a MW of 15,463 Da.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
Recombinant IL-21 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of human peripheral blood mononuclear cells is < 5ng/ml.
Accession No.: Q9HBE4
MW: 15463 Da
Sequence:
MQDRHMIRMR QLIDIVDQLK NYVNDLVPEF LPAPEDVETN CEWSAFSCFQ KAQLKSANTG NNERIINVSI KKLKRKPPST NAGRRQKHRL TCPSCDSYEK KPPKEFLERF KSLLQKMIHQ HLSSRTHGSE DS
Purity:
Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 10 ug protein lyophilized in 20 mM sodium phosphate pH7.5. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathway and Disease: Immune System, Signal Transduction, Signaling Molecules and Interaction
Alternate Names: interleukin-21, IL-21, Za11, IL21Description:
Interleukin-21 (IL-21), which is expressed by activated CD4(+) T cells, is the newest member of the common gamma-chain family of cytokines (IL-2, IL-4, IL-7, IL-9, IL-13, and IL-15). IL-21 receptor (IL-21R) is expressed on T, B, and NK cells. Within the B cell lineage, IL-21 regulates IgG1 production and cooperates with IL-4 for the production of multiple antibody classes in vivo. IL-21R has been shown to activate the Janus kinase/signal transducers and activators of transcription signaling pathway upon ligand binding. Initial studies have demonstrated that IL-21 has pleiotropic effects on the proliferation, differentiation, and effector functions of B, T, natural killer, and dendritic cells. Recombinant Human IL-21 produced in E. coli is a single non-glycosylated polypeptide chain containing 133 amino acids with a MW of 15,463 Da.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
Recombinant IL-21 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of human peripheral blood mononuclear cells is < 5ng/ml.
Accession No.: Q9HBE4
MW: 15463 Da
Sequence:
MQDRHMIRMR QLIDIVDQLK NYVNDLVPEF LPAPEDVETN CEWSAFSCFQ KAQLKSANTG NNERIINVSI KKLKRKPPST NAGRRQKHRL TCPSCDSYEK KPPKEFLERF KSLLQKMIHQ HLSSRTHGSE DS
Purity:
Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 10 ug protein lyophilized in 20 mM sodium phosphate pH7.5. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
| Is Featured? | No |
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