IL-3β polyclonal, anti-rat
€0.00
In stock
SKU
AC-ABG10277
Catalog Number: AC-ABG10277
Size(s): 50 µg, 100 µg
Host: Goat
Applications: WB, E
Datasheet
Request Information
Size(s): 50 µg, 100 µg
Host: Goat
Applications: WB, E
Datasheet
Request Information
Source:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant Rat IL-3beta. Anti-Rat IL-3beta specific antibody was purified by affinity chromatography employing immobilized Rat IL-3beta matrix.
Western Blot:
To detect Rat IL-3beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-3beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich ELISA:
To detect Rat IL-3beta by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Rat IL-3beta (62-003BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-3beta.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Rat IL-3beta (150 ng/ml), a concentration of 0.68 - 1.03 μg/ml of this antibody is required.
Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant Rat IL-3beta. Anti-Rat IL-3beta specific antibody was purified by affinity chromatography employing immobilized Rat IL-3beta matrix.
Western Blot:
To detect Rat IL-3beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-3beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich ELISA:
To detect Rat IL-3beta by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Rat IL-3beta (62-003BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-3beta.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Rat IL-3beta (150 ng/ml), a concentration of 0.68 - 1.03 μg/ml of this antibody is required.
Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
| Is Featured? | No |
|---|
Write Your Own Review