IL-6 monoclonal, anti-human
€0.00
In stock
SKU
AC-ABG10300
Catalog Number: AC-ABG10300
Size(s): 500 µg
Host: Mouse
Applications: WB, IHC, E
Datasheet
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Size(s): 500 µg
Host: Mouse
Applications: WB, IHC, E
Datasheet
Request Information
Source:
Produced in BALB/c mice using highly pure (>98%) recombinant human IL-6 as the immunizing antigen. This IgG1k antibody was purified from cell culture by Protein A affinity chromatography.
Western Blot:
To detect hIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-6 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.
Sandwich ELISA:
In a sandwich ELISA (assuming 100 μl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 500 pg/ml of recombinant human IL-6 when used with BioGems biotinylated antigen affinity purified anti-human IL-6 (60-006BT) as the detection antibody at a concentration of approximately 0.5-1.0 μg/ml.
Immunohistochemistry:
This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentration is 0.125 μg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
*Additional Immunostaining data available. Please contact Tech Support for information.
Formulation: A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Produced in BALB/c mice using highly pure (>98%) recombinant human IL-6 as the immunizing antigen. This IgG1k antibody was purified from cell culture by Protein A affinity chromatography.
Western Blot:
To detect hIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-6 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.
Sandwich ELISA:
In a sandwich ELISA (assuming 100 μl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 500 pg/ml of recombinant human IL-6 when used with BioGems biotinylated antigen affinity purified anti-human IL-6 (60-006BT) as the detection antibody at a concentration of approximately 0.5-1.0 μg/ml.
Immunohistochemistry:
This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentration is 0.125 μg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
*Additional Immunostaining data available. Please contact Tech Support for information.
Formulation: A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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