IL-7 human recombinant protein

Background:
IL7, as known as interleukin-7 isoform 1, is a cytokine of the hemopoietin family that plays important roles in lymphocyte differentiation, proliferation, and survival. This protein is a hematopoietic growth factor secreted by stromal cells in the bone marrow and thymus. It is also produced by keratinocytes, dendritic cells, hepatocytes, neurons, and epithelial cells but is not produced by normal lymphocytes. It stimulates the proliferation of lymphoid progenitors and is important for proliferation during certain stages of B-cell maturation. IL7 and HGF form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. It can be produced locally by intestinal epithelial and epithelial goblet cells and may serve as a regulatory factor for intestinal mucosal lymphocytes. Recombinant human IL7, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names:
Interleukin-7 isoform 1, IL7, IL-7, Il7, Interleukin-7

Domain: 26-177aa
Sequence:
DCDIEGK DGKQYESVLM VSIDQLLDSM KEIGSNCLNN EFNFFKRHIC DANKEGMFLF RAARKLRQFL KMNSTGDFDL HLLKVSEGTT ILLNCTGQVK GRKPAALGEA QPTKSLEENK SLKEQKKLND LCFLKRLLQE IKTCWNKILM GTKEH

UniProt No.: P13232
NCBI Accession No.: NP_000871.1

Molecular Weight: 18.4 kDa (161aa)
Concentration: 0.5mg/ml (determined by absorbance at 280nm)
Formulation: Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Purity: > 90% by SDS - PAGE
Bioactivity: Measured in a cell proliferation assay using PHA-activated human Peripheral Blood Mononuclear Cells(PBMC). The ED50 range ≤ 8 ng/ml.
Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
Endotoxin: His-Tag
Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.