IL12B (clone AT1D6), anti-human, rat

Decription:
IL-12 is a heterodimer composed of subunits IL-12A p35 and IL-12B p40. Autocrine IL-12 is responsible for the differentiation of naive CD4+T cells into type 1 helper T cells (Th1) that produce interferon-gamma (IFN-gamma). Expression of STAT 4, a signal transduction molecule important in cytokine-induced gene expression, appears central to IL-12 induced Th1 production of IFN-gamma. It also activates production of tumor necrosis factor alpha (TNFalpha) from T and natural killer cells. IL-12 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

Synonyms: Interleukin 12B precursor,interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40), CLMF, IL-12B, NKSF, CLMF2, natural killer cell stimulatory factor-2, cytotoxic lymphocyte maturation factor 2 p 40, interleukin 12 p40, natural killer cell stimulatory factor 40 kD subunit, interleukin-12 beta chain, IL12 subunit p40,

Clone: AT1D6. Anti-human IL12B mAb, clone AT1D6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human IL12B protein.

Isotype: Mouse IgG1, k

Immunogen: Recombinant human IL12B (23-328aa) purified from E. coli

Reactivity: human, rat

Form: Purified, in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Applications: ELISA, WB (Tissue, Cell lysate)

Working Dilution: Optimal dilution should be performed by user.

NCBI Nr.: NP_002178

Concentration: 0.1 mg/ml

Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.

References:
korman BD, et al., (2008) Curr Allergy Asthma Rep. 8(5):398-403
Holscher C., (2004) Med Microbiol Immunol. 193(1):1-17