iNOS polyclonal, anti-human, mouse

Background:
Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells. The inducible form of NOS, NOS2 (also designated iNOS), is Ca2+-independent and is expressed in a broad range of cell types.

Alternative Name:
HEP-NOS, Inducible NOS, iNOS, NOS2, NOS2A, HEP-NOS, Hepatocyte NOS, HEPNOS, inducible, Inducible nitric oxide synthase, Inducible NO synthase, Inducible NOS, iNOS, MAC NOS, Macrophage NOS, Nitric oxide synthase 2 inducible, Nitric oxide synthase 2 inducible macrophage, nitric oxide synthase 2A (inducible, hepatocytes), Nitric oxide synthase, Nitric oxide synthase inducible, nitric oxide synthase, macrophage, NOS 2, NOS, Nos II, NOS type II, nos2, NOS2, NOS2A, NOS2A, Inducible, Hepatocyte, Peptidyl-cysteine S-nitrosylase NOS2,

Application Dilution:
WB:1:500
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Specificity: iNOS polyclonal antibody detects endogenous levels of iNOS protein.

Immunogen:
Peptide

MW: 131 kDa

Swiss-Prot: P35228(Human) P29477(Mouse)

Purification & Purity:
Peptide affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

For research use only, not for use in diagnostic procedure.