Inositol triphosphate (IP3) detection kit

Use: Used to detect the concentration of inositol triphosphate (IP3) in samples such as serum, plasma, and cell culture supernatant.Specificity: The kit uses enzyme-linked immunosorbent assay. Biotin-labeled inositol triphosphate (IP3) is used, and the purified anti-inositol triphosphate (IP3) antibody is coated on the microplate. In the competitive inhibition reaction, a certain amount of solid-phase antibody and biotin-labeled inositol triphosphate (IP3) and non-labeled antigen (calibrator or specimen) inhibit the competitive reaction. The binding amount of antibody and biotin-labeled inositol triphosphate (IP3) is inhibited by the amount of non-labeled antigen. The more non-labeled antigen, the less binding of antibody and biotin-labeled inositol triphosphate (IP3), and vice versa. After the reaction is balanced, solid-phase antibody-biotinylated inositol triphosphate (IP3) is formed, and then enzyme-labeled avidin is added to form a solid-phase antibody-biotinylated inositol triphosphate (IP3)-enzyme label-avidin complex. After adding substrate for color development, the absorbance (OD value) is measured at a wavelength of 450nm using an enzyme reader. As the concentration of inositol triphosphate (IP3) increases, the OD value gradually decreases and shows a good linear relationship. This kit has the characteristics of high sensitivity, strong specificity, good repeatability, simple operation and rapidity, and has reliable detection performance for the reduction or increase of inositol triphosphate (IP3) in samples.

Storage Conditions
Store at 2-8°C.

Use: The kit is for research use only.