Insulin polyclonal, anti-human, pig, rat

Quantity: 100 µl

Background:
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants.

Synonyms:
INS, ILPR, IRDN, IDDM2, MODY10

Isotype: Rabbit Polyclonal

Immunogen:
Porcine Insulin

Form: Serum containing 0.1% sodium azide

Specificity:
The antibody can be used for the diagnosis of pancreatic B-cell tumors. Antigen. Localization: cytoplasm, extracellular.

Reactivity: human, pig, rat

Applications: Immunohistochemistry of frozen and paraffin-embedded sections; microwave treatment (citrate buffer) required. ELISA

Working DIlution: Dilute 1:25 -1:50 for immunohistochemistry

Positive Control:
Pancreas

Storage: 2-8C for immediate use, or at -20C (aliquot)

References:
- Luts A et al. (1989) Cell Tissue Res 258: 259-267