Insulin/Proinsulin polyclonal, anti-human, pig, rat, hamster

Quantity: 50 µl

Background:
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. Proinsulin is the prohormone precursor to insulin made in the beta cells of the islets of Langerhans, specialized regions of the pancreas. In humans, proinsulin is encoded by the INS gene.

Synonyms:
INS, ILPR, IRDN, IDDM2, MODY10

Isotype: Guinea Pig Polyclonal

Immunogen:
Synthetic human proinsulin

Form: Serum containing 0.1% sodium azide

Specificity:
Absorption with 10-100 ?g proinsulin per ml diluted antiserum inactivates the antiserum, while C-peptide and insulin only partly reduce staining.

Reactivity: human, pig, rat, hamster

Applications: Immunohistochemistry, suitable for frozen and paraffin sections

Working DIlution: 1:300 - 1:500 PAP; 1:100 – 1:200 Immunofluorescence microscopy using FITC

Positive Control:
Formalin-fixed paraffin sections of human pancreas

Storage: 2-8C for immediate use, or at -20C (aliquot)

References:
- Myrsén et al. (1996) Diabetes 45: 1306-1313
- Kohnert KD et al. (1999) Regul Pept 82: 71-79
- Ahre?n B et al. (1999) Pancreas 18: 75-83
- Myrse?n-Axcrona U et al. (1996) Diabetes 45: 1306-1313