Integrin β1 (Extracellular region) (clone M032), anti-human

Background:
Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin receptors contain noncovalently associated α and β subunits that consist of a large extracellular region (the ligand-binding domain), a short transmembrane region, and a cytoplasmic domain of varying length. In mammals, at least 17 α subunits and 8 β subunits have been identified and these proteins can heterodimerize to form at least 22 different receptors. The β1 subfamily includes 12 distinct integrin proteins that bind to different extracellular matrix molecules. Integrin β1 has been implicated in various activities including embryonic development, blood vessel, skin, bone, and muscle formation, as well as tumor metastasis and angiogenesis.

Background References
Wang, L. et al. (2012) J Cell Physiol. 227(2):474.
Iwamoto, D. & Calderwood, D. (2015) Cur Opin Cel Bio. 36:41.

Immunogen: Clone M032 was generated from a proprietary antigen related to the extracellular region of human integrin β1 expressed in A431 epidermoid carcinoma cell line.

Specificity: Clone M032 detects a 120-150 kDa* band corresponding to Integrin β1 on SDS-PAGE immunoblots of "Native" human A431, A549, MDA-MB-231, and LNCaP cell lysates, as well as human breast, skin, and lung tissues. The antibody weakly detects the denatured form of Integrin β1. Clone M032 can be used in western blot, immunocytochemistry, and ELISA.

Application dilution:
ELISA: 1:2000
ICC: 1:200
WB: 1:500
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.

Buffer/Storage:
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100 µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.