Integrin Beta4 (Cytoplasmic Region) (clone M126), anti-human

Background:
Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin α6β4receptors are found in basement membrane along with laminin-5. These receptors are expressed in epithelial, schwann, endothelial and some immune cells. The cytoplasmic domain of the Integrin β4 subunit recruits the adaptor protein Shc and is required for assembly of hemidesmosomes. Tyrosine phosphorylation of multiple sites within the cytoplasmic domain regulates these cellular events. In particular, tyrosine 1526 interacts with the phosphotyrosine binding domain of Shc and is required for Shc activation. In addition, tyrosine 1494 is required for integrin-mediated IRS-2 phosphorylation and activation of PI3-kinase. More importantly this site is critical for integrin α6β4 increases in carcinoma invasion.

Immunogen: Clone M126 was generated from a recombinant protein containing amino acid residues in the cytoplasmic region of human Integrin β4. This sequence is found in all three Integrin β4 isoforms and has 90% homology with rat and mouse Integrin β4.

Specificity: This antibody detects a 200kDa* protein corresponding to the molecular mass of Integrin β4 on SDS-PAGE immunoblots of human A431 cells.

Buffer/Storage:
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.