Interleukin-33 (IL-33) (clone 4E9 ), anti-human

Decription:
Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine and intracellular nuclear factor with transcriptional regulatory properties. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kDa and C-terminal fragment of 18kDa by cleavage of caspase-1.

Synonyms: Interleukin 33, DVS27, NF-HEV, NKHEV, C9orf26, IL-33, Interleukin 33 CHROMOSOME 9 OPEN READING FRAME 26, DKFZp586H0523, DVS27 related protein, IL 1F11, IL 33, IL1F11, Interleukin 1 family member 11, INTERLEUKIN 33 NFHEV, Interleukin 33 precursor, Interleukin33, NF HEV, NFHEV, Nuclear factor from high endothelial venules, RP11 575C20.2, il33

Clone: 4E9. Anti-human IL-33 mAb, clone 4E9, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human IL-33 protein.

Isotype: Mouse IgG2b kappa

Immunogen: Recombinant human IL-33 (112-270aa) purified from E. coli

Reactivity: human

Form: Purified, in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Applications: ELISA, WB (Cell lysate)

Working Dilution: Optimal dilution should be performed by user.

NCBI Nr.: NP_254274

Concentration: 0.1 mg/ml

Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.

References:
Brint Ek, et al., (2005) J Biol Chem. 20:277(51)
Schmitz, et al., (2005) immunity. 23:479
Baekkevold ES, et al., (2003) Am.J.Pathol. 163(1):69-90