Interleukin-33 (IL-33) (clone IL33026B), anti-human, mouse

Decription:
Interleukin-33 (IL-33; HF-NEV; IL-1F11), a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30kDa human IL33 is converted by CASP1 to a 18kDa protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1(MAPK3)/ERK2(MAPK1), p38(MAPK14), and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.

Synonyms: Interleukin-33, IL-1F11, NF-HEV

Clone: IL33026B

Isotype: Mouse IgG1 kappa

Immunogen: Recombinant human IL-33.

Reactivity: human, mouse

Specificity: Recognizes human and mouse IL-33.

Form: Purified, in 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Applications:
ELISA: (direct or indirect: 1:2'000-1:5'000) Immunoprecipitation
Western blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature).

Working Dilution: Optimal conditions must be determined individually for each application.

Concentration: 1mg/ml

Storage: 2-8C immediate use, or -20C (aliquote)