Involucrin (Squamous Cell Terminal Differentiation Marker) (clone SPM259), anti-human, gorilla, Owl monkey, pig, and dog
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In stock
SKU
ARP-24-3713-MSM1X
Catalog Number: 24-3713-MSM1X
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG1, kappa
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG1, kappa
Decription:
It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this monoclonal antibody reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
Synonyms: IVL
Clone: SPM259
Isotype: Mouse IgG1, kappa
Immunogen: Purified involucrin from human keratinocytes
Reactivity: human, gorilla, Owl monkey, pig, and dog. Does not react with mouse. Others not known.
Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applications: Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistology (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37 �C)
(Note than enzyme digestion is better than citrate buffer based epitope unmasking)
Optimal dilution for a specific application should be determined.
Reactivity: MCF-7 cells. Localized to upper spinous and granular layers in normal skin.
Concentration: 0.2mg/ml
Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this monoclonal antibody reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
Synonyms: IVL
Clone: SPM259
Isotype: Mouse IgG1, kappa
Immunogen: Purified involucrin from human keratinocytes
Reactivity: human, gorilla, Owl monkey, pig, and dog. Does not react with mouse. Others not known.
Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applications: Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistology (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37 �C)
(Note than enzyme digestion is better than citrate buffer based epitope unmasking)
Optimal dilution for a specific application should be determined.
Reactivity: MCF-7 cells. Localized to upper spinous and granular layers in normal skin.
Concentration: 0.2mg/ml
Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
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