IQGAP1 polyclonal, anti-human, mouse

Background:
IQGAP1, for IQ motif containing GTPase activating protein, is a Ras GAP-related, Actin-binding protein that interacts with the small GTPases Cdc42 and Rac1. The C-terminus of IQGAP1 is essential for interacting with Cdc42 and, in addition, IQGAP1 contains a WW domain and a predicted N-terminal coiled-coil region, which may be involved in IQGAP dimerization. Expression of IQGAP1 is highest in placenta, lung and kidney, where it co-localizes with Cdc42 to the cytoskeleton and assists with Cdc42 in mediating the regulation of cell proliferation, polarity and cell morphology. IQGAP1 regulates cadherin-mediated cell adhesion via binding to E-cadherin, b-catenin and a-catenin. This association induces the accumulation of these proteins at the site of cell-cell contact. IQGAP1 is negatively regulated by calmodulin, which binds to IQGAP1 in a calcium-dependent manner and disrupts IQGAP1 from associating with Cdc42.

Alternative Name:
IQGAP1, KIAA0051, HUMORFA01, IQ motif containing GTPase activating protein 1, IQGA1, Iqgap1, KIAA0051, p195, Ras GTPase activating like protein 1, Ras GTPase-activating-like protein IQGAP1, RasGAP-like with IQ motifs, SAR1,

Application Dilution:
WB:1:500-1:2,000
ICC/IF:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Specificity: IQGAP1 polyclonal antibody detects endogenous levels of IQGAP1 protein.

Immunogen:
Recombinant protein corresponding to N-terminal human IQGAP1.

MW: 189 kDa

Swiss-Prot: P46940(Human) Q9JKF1(Mouse)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.