JE/MCP-1 polyclonal biotin, anti-mouse
€0.00
In stock
SKU
AC-ABG10325
Catalog Number: AC-ABG10325
Size(s): 25 µg, 50 µg
Host: Rabbit
Applications: WB, E
Datasheet
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Size(s): 25 µg, 50 µg
Host: Rabbit
Applications: WB, E
Datasheet
Request Information
Source:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mJE(MCP-1). Anti-Murine JE(MCP-1) specific antibody was purified by affinity chromatography and then biotinylated.
Western Blot:
To detect mJE(MCP-1) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mJE(MCP-1) is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich ELISA:
To detect mJE(MCP-1) by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 / 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems Polyclonal Anti-Murine JE(MCP-1) (61-083P) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mJE(MCP-1).
Direct ELISA:
To detect mJE(MCP-1) by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 / 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mJE(MCP-1).
Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mJE(MCP-1). Anti-Murine JE(MCP-1) specific antibody was purified by affinity chromatography and then biotinylated.
Western Blot:
To detect mJE(MCP-1) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mJE(MCP-1) is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich ELISA:
To detect mJE(MCP-1) by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 / 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems Polyclonal Anti-Murine JE(MCP-1) (61-083P) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mJE(MCP-1).
Direct ELISA:
To detect mJE(MCP-1) by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 / 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mJE(MCP-1).
Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
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