Jurkat Camptothecin Control Lysate
€175.00
In stock
SKU
ECM-JL9531
Background:
The camptothecin treatment of human Jurkat T-Cell leukemia cells can be used as a western blot positive control for detecting antigens expressed during apoptosis. The treated cells undergo apoptosis as determined by identification of specific apoptotic cleavage products, such as PARP and caspase-3 cleavage. Confluent cultures of Jurkat cells were either left untreated (Cat.# JL9531) or treated with Camptothecin (4 μM) for 4 hours at 37°C (cat.# JL9541). Cells were lysed in 1% SDS, 1.0 mM sodium ortho-vanadate, 1 mM sodium fluoride in 10 mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.
Buffer/Storage:
Cell Lysates are supplied at a concentration of 1 mg/ml in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.
The camptothecin treatment of human Jurkat T-Cell leukemia cells can be used as a western blot positive control for detecting antigens expressed during apoptosis. The treated cells undergo apoptosis as determined by identification of specific apoptotic cleavage products, such as PARP and caspase-3 cleavage. Confluent cultures of Jurkat cells were either left untreated (Cat.# JL9531) or treated with Camptothecin (4 μM) for 4 hours at 37°C (cat.# JL9541). Cells were lysed in 1% SDS, 1.0 mM sodium ortho-vanadate, 1 mM sodium fluoride in 10 mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.
Buffer/Storage:
Cell Lysates are supplied at a concentration of 1 mg/ml in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.
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