KDEL polyclonal, anti-human, mouse, rat

Background:
Soluble proteins in the endoplasmic reticulum (ER) contain a specific carboxy terminal sequence KDEL (Lys-Asp-Glu-Leu), and include the coat proteins required for vesicle budding from the ER, proteins that form retrograde vesicles on post-ER compartments, and integral membrane proteins that target vesicles to their correct destination. The retention of these soluble proteins in the ER depends on the interaction of the KDEL sequence with the corresponding KDEL receptor, also designated ERD2, in the Golgi apparatus. When KDEL proteins reach the Golgi complex, they are recognized by the KDEL receptor and transported retrograde in COPI-coated vesicles back to the ER. The small GTPase ADP-ribosylation factor 1 (ARF1), a regulator of vesicle transport, interacts with the KDEL receptor. Subsequently, this interaction allows the KDEL receptor to recruit a GTPase-activating protein (GAP) from the cytosol to membranes, which inactivates ARF1.

Alternative Name:
KDEL receptor 1, KDELR1, ERD2.1, ER lumen protein retaining receptor 1, ERD2.1, ERD21, KDEL endoplasmic reticulum protein retention receptor 1, KDEL receptor 1, Kdelr1, Putative MAPK-activating protein PM23,

Application Dilution:
WB:1:500-1:2000
ICC:1:400-1:800
IHC:1:100-1:400
FC:1:50-1:100

Specificity: KDEL polyclonal antibody detects endogenous levels of KDEL protein.

Immunogen:
Recombinant protein

MW: 24/17 kDa(Predicted band size)

Swiss-Prot: P24390(Human) Q99JH8(Mouse) Q569A6(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.