Keratin 18 (clone EBS-IF-006), anti-human, cat, dog, pig

Source:
A hybrid mouse (BALB/c x B6) was immunized with a cytokeratin-enriched extract from A431 cells (human vulvar squamous carcinoma).
Fusion partner: Sp2/0.

Specifications:
EBS-IF-006 reacts in immunoblotting specifically with human keratin 18 (45kDa). In tissue sections EBS-IF-006 is positive with normal epithelia and carcinomas expressing keratin 18, i.e. simple, transitional and glandular epithelia and basal cells of non-cornifying stratified epithelia (ectocervix, esophagus), adenocarcinomas of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid and some squamous cell carcinomas. EBS-IF-006 was typed in the ISOBM TD-5 workshop.

Species reactivity:
Positive: cat, dog, human, pig. Negative: ferret, rat.

Applications:
Demonstration of keratin 18 in immunohistochemistry, immunofluorescence tests and immunoblotting.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate
Western blot: +

Positive control: Skin.

References:
- Ivanyi D, et al. J. Pathol. 159: 7-12 (1989).
- Ivanyi D, et al. Differentiation. 42(2): 124-129 (1989).
- Ivanyi D, et al. Am. J. Vet. Res. 53: 304-314 (1992).
- Vos et al. Vet.Pathol. 30: 271-279 (1993).
- Vos et al. Vet.Pathol. 30: 352-361 (1993).
- Balm A.J.M, Eur. Arch. Otorhinolaryngol.253: 227-233 (1996).
- Nap, M, et al. Tumor Biology19: 4-10, (1998).
- Stigbrand, T, et al. Tumor Biology19: 132-152, (1998)