Keratin 19 (clone EBS-IF-007), anti-human

Source:
A BALB/c mouse was immunized with human breast cancer cell line MCF-7.
Fusion partner: P3-X63-Ag8.653.

Specifications:
EBS-IF-007 is human specific and reacts with rod domain 2B, aa 311-335, of keratin 19 (40 kDa). Keratin 19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-keratin 19 reacts with a wide variety of corresponding epithelial malignancies.

Species reactivity:
Positive: human. Negative: ferret, rat.

Applications:
EBS-IF-007 can be used on paraffin sections using either citrate or protease pre-treatment.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate/protease
Western blot: +

Positive control: MCF-7, mesothelia, urothelium, and bile duct epithelium, breast, lung.

References:
- TD-5 Workshop Report, Tumor Biology19. (1998).
- Böttger, V. et al., Eur. J. Biochem.231: 475-485 (1995)
- Karsten et al., Eur.J.Cancer Clin. Oncol. 21(6), 733-740 (1985).
- Kasper et al., Eur.J.Cancer Clin. Oncol. 23(2), 137-147 (1987).
- Kasper et al., Histochemistry89(4), 369-377 (1988).
- Kasper and Karsten, Cell Tissue Res. 253(2), 419-424 (1988).
- Kasper et al., Differentiation40, 207 (1989).
- Stosiek et al., Liver10, 59 (1990).
- Karsten et al., Differentiation54, 55 (1993).
- Kovařík et al. Int.J.Cancer, Suppl. 3: 50-55 (1988).