Keratin 7 + 17 (clone C-46), anti-human, bovine, dog, pig, sheep

Source:
A hybrid mouse (BALB/c x B6) was immunized with purified human keratin 7, isolated from HeLa cytoskeleton.
Fusion partner: P3-X63-Ag8.653.

Specifications:
C-46 reacts with keratins 7 (54kDa) and 17 (46kDa). On formalin-fixed paraffin embedded sections C46 reacts with keratin 7 only.Strongly positive on most simple epithelia except for stomach, small intestine and colon mucosa, hepatocytes, pancreatic acini, renal tubules, also positive on some non-cornifying epithelia.

Species reactivity:
Positive: cow, dog, human, pig, sheep. Negative: ferret, mouse, rabbit, rat.

Applications:
Demonstration of keratins 7 and 17 in a variety of species, using IHC, IF and WB.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Pepsin
Western blot: +

Positive control: Relevant simple epithelia like ovary, lung, and breast.

References:
- Bártek et al. J. of Pathol.164: 215-24 (1991).
- Vojtĕšek et al. Neoplasma37(3): 333-342 (1990).
- Taylor –Papadimitriou J. et. al. J. Cell Sci.94: 403-13 (1989).
- Kovařík et al. Int.J.CancerSuppl. 3: 50-55, (1988).
- Kovařík et al. J. Tumor Marker Oncol.5: 219, (1990).