Keratin K10 polyclonal, anti-human, mouse, rat

Quantity: 25 µl

Background:
This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21.

Synonyms:
KRT10, BIE, EHK, K10, KPP, BCIE, CK10

Isotype: Guinea pig Polyclonal (GPK10.1)

Immunogen:
Synthetic peptide of human keratin K10 (formerly alsodesignated cytokeratin 10; C-GS VGE SSS KGP RY), coupledto KLH

Form: Stabilized Serum

Specificity:
MW 56,5000 (pI 5.3) intermediate filament polypeptide, keratin K10, detected by immunohistochemistry in the suprabasal layers of human epidermis (basal cells are negative). Useful for the recognition of keratinizing cells in squamous cell carcinoma of epidermis, lung, bladder, cervix, esophagus etc. Completely negative on non-stratified epithelia.

Reactivity: human, mouse, rat

Applications: Suitable for immunofluorescence microscopy on frozen sections, immunohistochemistry on paraffin sections (after microwave treatment or trypsin), Western blotting

Working DIlution: 1: 100-1:200 for immunohistochemistry

Positive Control:
Human suprabasal keratinocytes of epidermis; squamous cell carcinoma

Storage: 2-8C

References:
- Langbein L et al. (2006) J Invest Dermatol 126: 2377-2386