Keratin K12 polyclonal, anti-human, pig, mouse

Quantity: 25 µl

Background:
KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy.

Synonyms:
KRT12, K12

Isotype: Guinea pig Polyclonal (GPK12.2)

Immunogen:
Synthetic peptide of human keratin K12 (formerly alsodesignated cytokeratin 12; DLS NNT MSL SVR T-C), coupledto KLH

Form: Stabilized Serum

Specificity:
MW 55,000 (pI 4.9) intermediate filament polypeptide, keratin K12, detected by immunohistochemistry in the cornea only. Useful as a marker for corneal type differentiation (positive for epithelial cells of cornea) and for studying corneal epithelial stem cells.

Reactivity: human, pig, mouse

Applications: Suitable for immunofluorescence microscopy on frozen sections, immunohistochemistry on paraffin sections (after microwave treatment or trypsin)

Working DIlution: 1: 100-1:200 for immunohistochemistry

Positive Control:
Human cornea

Storage: 2-8C

References:
- Langbein L et al. (2006) J Invest Dermatol 126: 2377-2386