Keratin K13 polyclonal, anti-human, mouse

Quantity: 25 µl

Background:
The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been described.

Synonyms:
KRT13, K13, CK13

Isotype: Guinea pig Polyclonal (K13.2)

Immunogen:
Synthetic peptide (TTTSSASVTTTSNA-C) of human cytokeratin 13, conjugated to KLH

Form: Stabilized Serum

Specificity:
Acidic human keratin K13 (Mr 55 000). Tumors specifically detected: several squamous cell carcinomas, e.g. cervix carcinoma; transitional cell carcinoma of the bladder; craniopharyngioma

Reactivity: human, mouse

Applications: Immunohistochemistry on frozen and paraffin sections (after microwave treatment), Immunoblotting (western, ECL), Cytological material

Working DIlution: 1:200 for frozen sections; 1:100 for paraffin sections; 1:3000 for Immunoblotting

Positive Control:
Squamous epithelium of esophagus, gingival, palate, vagina (staining all of the suprabasal layers)

Storage: 2-8C

References:
- Moll, R. et al. (1982) Cell 31: 11-24