Keratin K16 polyclonal, anti-human, mouse

Quantity: 25 µl

Background:
The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2.

Synonyms:
KRT16, K16, CK16, K1CP, NEPPK, FNEPPK, KRT16A

Isotype: Guinea pig Polyclonal (GPK16.1)

Immunogen:
Synthetic peptide of human keratin K16 (formerly alsodesignated cytokeratin 16; C-KE QSS SSF SQG QSS), coupledto KLH

Form: Stabilized Serum

Specificity:
MW 48,000 (pI 5.1) intermediate filament polypeptide, keratin K16, detected by immunohistochemistry in psoriatic skin, in the rete ridges of foot sole epidermis, in tongue, esophagus, exocervix and in squamous cell carcinoma of skin, tongue and cervix. In the hair follicle, staining was restricted to the outer root sheath. Completely non-reactive were cells of non-stratified epithelia (e.g. liver, colon).

Reactivity: human, mouse

Applications: Suitable for immunofluorescence microscopy on frozen sections, immunohistochemistry on paraffin sections (after microwave treatment)

Working DIlution: 1: 100-1:200 for immunohistochemistry

Positive Control:
Rete ridges of human foot sole epidermis, psoriatic skin

Storage: 2-8C

References:
- Langbein L et al. (2006) J Invest Dermatol 126: 2377-2386