Keratin K18 polyclonal, anti-human, mouse

Quantity: 25 µl

Background:
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

Synonyms:
K18, CYK18

Isotype: Guinea pig Polyclonal (K18-2.2)

Immunogen:
Synthetic peptide from human keratin K18 (CSYGARPVSSAASVY)

Form: Stabilized Serum

Specificity:
Human type I (acidic) keratin K18 (Mr 45,500; formerly also designated cytokeratin 18). The antiserum represents an excellent marker for all simple (non-stratified) epithelia and basal cells of many squamous, non-cornified epithelia. No cross-reaction with other keratins and intermediate filament proteins. Tumors Specifically Detected: All adeno-Ca tested, undifferentiated CA, hepatocellular CA, cervix Ca.

Reactivity: human, mouse

Applications: Suitable for immunohistochemistry on frozen and paraffin-embedded cells and tissue (after microwave treatment), immunoblotting (Western)

Working DIlution: 1:200 for immunohistochemistry; 1:100 for paraffin-embedded tissue; 1:2000 for Western blotting (ECL)

Storage: 2-8C

References:
- Langbein L et al. (2006) J Invest Dermatol 126: 2377-2386