Keratin K20 polyclonal, anti-human, mouse

Quantity: 25 µl

Background:
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.

Synonyms:
K20, CD20, CK20, CK-20, KRT21

Isotype: Guinea pig Polyclonal (K20.2)

Immunogen:
Synthetic peptide (KTT EYQ LST LEE RD-C) of human keratin K20 (formerly also designated cytokeratin 20), coupled to KLH

Form: Stabilized Serum

Specificity:
Acidic human keratin K20 (Mr 46 000). Very sensitive detection of intestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma).

Reactivity: human, mouse

Applications: Immunohistochemistry on frozen and paraffin (after microwave treatment or trypsin) sections, Immunoblotting (western, ECL), Cytological material

Working DIlution: 1:100 paraffin sections; 1:200 frozen sections; 1:3000 Immunoblotting

Positive Control:
Colon, duodenum, Merkel cells of skin

Storage: 2-8C

References:
- Moll, R. et al. (1982) Cell 31: 11-24