Keratin K27 (K25irs3) polyclonal, anti-human

Quantity: 25 µl

Background:
This gene encodes a member of the type I (acidic) keratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. The type I keratin genes are clustered in a region of chromosome 17q12-q21.

Synonyms:
KRT27, KRT25C, K25irs3

Isotype: Guinea pig Polyclonal (CK M5-2.1)

Immunogen:
Synthetic peptide of human keratin K27 (formerly alsodesignated keratin K25irs3); aa 404-416 (GYG GPG NQTKDS S-C), coupled to KLH

Form: Stabilized Serum

Specificity:
Specific for human irs-specific keratin K27. The antiserum stains specifically the Henle, Huxley and cuticle layer of the inner root sheath (irs) of the hair follicle. Negative on all other epithelia tested.

Reactivity: human (weak cross-reaction with mouse)

Applications: Suitable for immunohistochemistry on frozen sections

Working DIlution: 1 : 100 - 200 for immunohistochemistry

Storage: 2-8C

References:
- Langbein L et al. (2006) J Invest Dermatol 126: 2377-2386