Keratin K3 polyclonal, anti-human, mouse

Quantity: 25 µl

Background:
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the corneal epithelium with family member KRT12 and mutations in these genes have been associated with Meesmann's Corneal Dystrophy. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Synonyms:
KRT3, K3, CK3

Isotype: Guinea pig Polyclonal (GPK3.2)

Immunogen:
Synthetic peptide of human keratin K3 (formerly alsodesignated cytokeratin 3; C- IKF SQS SQS SQR YSR), coupled to KLH

Form: Stabilized Serum

Specificity:
MW 63,000 (pI 7.5) intermediate filament polypeptide, keratin K3, detected by immunohistochemistry in the cornea only. Useful as a marker for corneal type differentiation (positive for epithelial cells of cornea) and for studying corneal epithelial stem cells.

Reactivity: human, mouse

Applications: Suitable for immunofluorescence microscopy on frozen sections

Working DIlution: 1: 100-1:200 for immunohistochemistry

Positive Control:
Human cornea

Storage: 2-8C

References:
- Langbein L et al. (2006) J Invest Dermatol 126: 2377-2386