Keratin K4 polyclonal, anti-human, mouse

Quantity: 25 µl

Background:
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in differentiated layers of the mucosal and esophageal epithelia with family member KRT13. Mutations in these genes have been associated with White Sponge Nevus, characterized by oral, esophageal, and anal leukoplakia. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Synonyms:
KRT4, K4, CK4, CK-4, CYK4

Isotype: Guinea pig Polyclonal (K4-3.2)

Immunogen:
Synthetic peptide (C-KIISTTTLNKRR) of human keratin K4coupled to KLH

Form: Stabilized Serum

Specificity:
Human type II (basic) keratin K4 (Mr 59 000), formerly als designated cytokeratin 4. Recognizes keratin K4 in non-cornifying squamous epithelia. Keratin K4 is mostly present in combination with keratin K13. In contrast to keratin K13, the superficial cells of the cornea are reactive with keratin K4. The antibody also stains ciliated pseudo-stratified epithelium of bronchi and, focally, ductal epithelial cells.

Reactivity: human, mouse

Applications: Suitable for Immunohistochemistry on frozen and paraffin sections, Immunoblotting (Western), Cytological material

Working DIlution: 1:200 for immunohistochemistry; 1:100 for paraffin-embedded tissue; 1:3000 for Western blotting (ECL)

Positive Control:
Squamous epithelium of esophagus, gingiva, palate, vagina (staining all suprabasal layers)

Storage: 2-8C

References:
- Langbein L et al. (2006) J Invest Dermatol 126: 2377-2386